What Is Rigor Mortis and How Do Forensic Experts Use It To Determine Time Of Death?

Rigor Mortis is the post-mortem stiffening of muscle. It takes 2 to 6 hours to develop and can last for 24 to 84 hours. It is useful to calculate PMI.

One of the most commonly heard words in crime dramas is rigor mortis. The forensic expert will somberly explain to the cops that the victim died more than 6 hours before the body was found, because they observed rigor mortis in the corpse. However, what does rigor mortis mean? And how do forensic experts use it to determine the time of death?

What is Rigor Mortis?

Rigor mortis is the stiffening of the body after death and is the third post-mortem sign of death. The first two are pallor mortis and algor mortis, respectively. Rigor mortis starts 2 to 6 hours after death and peaks approximately 12 hours after death.

These postmortem signs develop due to certain physiological changes that occur in the body after death. A forensic expert can use these changes to calculate the time of death. Time of death is more formally called the post-mortem interval, which is defined as the time interval from death to when the corpse was found.

Why does Rigor Mortis occur?

In 1811, a young French physician, P.H. Nysten, was studying guillotine victims. In what can only be imagined as dark-bricked and macabre rooms, Nysten experimented and observed criminals decapitated by the guillotine immediately after their death.  He noticed an odd stiffness in the muscles of criminals that progressed through the body downward with time, starting with the jaw. Although Nysten was the first to formally describe rigor mortis, he couldn’t uncover why it was happening. It was only later, in the 1900s, that the fascination with rigor mortis bore any fruit. Many researchers, E.C. Bate Smith, J.R. Bendall and Szent-Györgyi, just to name a few, were trying to uncover the physiological basis of rigor mortis. Slowly the jigsaw pieces of how and why rigor mortis occurs began to fall into place as scientists came to understand more about how muscles contract on a molecular level, and how those interactions change after death.

Jacques-Louis David - La Mort de Marat

The Death of Marat by French painter Jacques-Louis David in 1793. Rigor Mortis can be seen here by how the subject clutches paper and quill. (Photo Credit : public domain/Wikimedia Commons)

Rigor Mortis doesn’t start immediately after death. Rather, all the muscles in the body relax during a stage called primary flaccidity. After approximately 2 to 6 hours, rigor mortis sets in, which peaks about 12 hours after death, but can last 24 to 84 hours post-mortem. After rigor mortis comes another (final) wave of muscle relaxation called secondary flaccidity. These stages of death are caused by certain physiological changes occurring in muscle tissues.

Primary flaccidity

Muscle contraction occurs on command from the brain. The brain signals a muscle to contract through the motor neurons, which in turn signal the muscles (by releasing the neurotransmitter acetylcholine). A series of changes occur in the muscles that make them contract. After death, in the absence from that signal from the brain, muscles begin to relax.

Muscle relaxation during primary flaccidity follows a pattern that Nysten observed with rigor mortis. The first muscles to relax are those in the face, most notably the eyelids, and the muscles that control the fingers. The muscles in the upper body relax earlier, and are then followed by the lower body. In this stage, the corpse will also pass urine and release gases, as the muscles that control these processes relax.

Rigor Mortis

Before we can understand how rigor mortis occurs, it’s important to understand how muscles contract in the first place.

A muscle contraction is done with the help of two special protein filaments called actin filaments and myosin filaments. Myosin pulls the actin filaments, which causes contraction. Think of it as someone pulling a rope to bring something closer. The picture of a muscle fiber, called a sarcomere, shown below should make the process clearer.

However, myosin also requires energy to pull the actin. It gets this energy from ATP, the energy currency of the cell. ATP is short for adenosine triphosphate, which means that it is an adenosine molecule with three phosphate ions linked to it. Removing a phosphate from ATP releases energy, which the cell uses to do work.

Contraction starts when an ATP molecule is broken down into ADP and phosphate. This gives the myosin energy to “stand up” and bind (or grab on) to actin. This binding between the actin and myosin is called a cross-bridge (like bridging two molecules). Once bound, myosin pulls the actin, shortening the muscle fiber slightly.

Detail of a muscle sarcomere showing thin and thick filaments and mechanism of mechanical contraction(Blamb)s

The process of muscle contraction. The first panel shows a myofibril or a muscle fiber. A sarcomere is one contractile unit of the muscle fiber. The sarcomere is made of myosin (the thick red line in the middle) and actin (the thinner green lines). The myosin pulls the actin inwards making the sarcomere shorter. This is how muscle contraction works. The last panel shows exactly how myosin and actin interact. (Photo Credit : Blamb/Shutterstock)

One single round of myosin pulling actin doesn’t cause a muscle to contract. This must happen multiple times for a full contraction. This means that myosin must detach from the actin filament and initiate a new round of pulling. For this, a new molecule of ATP must bind to myosin. This causes the myosin to leave the actin molecule. The ATP molecule is broken down (hydrolyzed) into ADP and phosphate, restarting the process.

Rigor mortis occurs because of two factors—a lack of ATP, which prevents myosin from detaching from actin, as well as the release of calcium from a compartment in the muscle cell as a result of autolysis. We’ll discuss the latter factor before we delve into ATP.


Autolysis is the body breaking itself down, also known as cellular decay. It begins to occur minutes after death as a result of oxygen levels depleting. This activates certain cellular mechanisms that break down proteins and other macromolecules (like DNA and fats) in the cells of the body. As this happens, the compartments (organelles) within the cell are broken down, releasing their contents into the cytosol (the general fluid of the cell). One such event is the breakdown of the smooth endoplasmic reticulum (or sarcoplasmic reticulum, SR for short) in the muscle. The SR is a separate compartment in the muscle cell that stores calcium, along with various other molecules.

Role of calcium ions

Calcium ions are essential to start the contraction in the first place. In normally functioning muscles, calcium is released from the SR only when the brain sends a signal through the neurons. When calcium is released into the cytosol, it binds to a protein called troponin. Troponin, along with another associated protein, tropomyosin, are the gatekeepers of muscle contraction. They sit on actin filaments, covering up the parts of actin to which the myosin molecule binds.

When calcium binds to troponin, it causes troponin and tropomyosin to change its shape slightly so that they no longer cover the myosin-binding site of actin. The gates have been opened and myosin can finally bind to actin.

The second factor—the lack of free ATP—sustains rigor mortis for hours, but rigor mortis doesn’t set in immediately after primary flaccidity, and the release of calcium. There is a short delay where the muscles still have ATP molecules that allow the myosin to detach from actin.

The muscles have a reserve of ATP (in the form of a protein called creatine), as well as reserves of glucose in the form of glycogen (a bunch of glucose connected in a long branching string).

Even after the supply of oxygen to the muscle is gone, the muscles can still make ATP by anaerobically (in the absence of oxygen) breaking down glucose. This is what happens when you do intense exercise and the muscles don’t have time to wait for oxygen to make more ATP. With these two reserves in place, the muscles can relax back.

However, once these ATP reserves run out, the myosin can no longer detach from actin. When this happens and more myosin heads attach to actin, rigor mortis reaches its peak.

Like the French physician Nysten elucidated with his guillotined criminals, rigor mortis progresses downward, starting from the face and ending with the legs. This may appear to be true, although a case was later made that rigor mortis starts almost simultaneously in every muscle; however, because of different thicknesses of muscle, some take longer to reach full rigor than other. The muscles in the face, such as the eyelids and jaw, are smaller and have fewer muscle fibers. The muscles in the torso and thighs are larger and would take more time before becoming completely contracted.

Secondary Flaccidity

Rigor mortis doesn’t go on ad infinitum. Cellular degradation eventually catches up and begins to break down the actin and myosin proteins themselves. This causes a second round of relaxation called secondary flaccidity, which occurs in the same order as primary flaccidity and rigor mortis.

This is a complex set of processes, so here is a short bullet-point summary:

  • After death, muscles relax due to the lack of a signal from the nervous system to contract, as well as the breakdown of ATP and glucose reserves. This is called primary flaccidity.
  • After primary flaccidity, rigor mortis takes place.
  • Calcium is released from its storage compartments in the cell due to cellular degradation, which initiates muscle contraction.
  • The muscles run out of glucose and ATP, which is needed for the muscles to relax. Without ATP, the muscles remain contracted. This is rigor mortis. It begins 2 to 6 hours after death and can last 24-84 hours after death.
  • Cellular degradation begins breaking down the muscles (especially the actin and myosin proteins responsible for muscle contraction). This causes the muscles to once again relax in a stage called secondary flaccidity.

How do forensic experts use rigor mortis to determine the time of death?

Rigor mortis is a fairly reliable method to estimate time of death. Post-mortem interval (PMI), as the time of death is known formally, is the time between death and when the body was discovered. Forensic experts use many different methods to determine PMI, rigor mortis being one of the most popular approaches.

The progression of rigor mortis is predictable and isn’t affected by external conditions. Temperature, however, does influence how fast rigor mortis progresses. At lower temperatures, rigor sets in faster and is prolonged for longer periods. The onset of rigor mortis is quicker if an individual has recently exercised or done any other vigorous activity.

Once the forensic expert knows about these other external conditions and any other evidence, they can use rigor mortis to approximate a victim’s time of death.


  1. Guyton and Hall Textbook of Medical Physiology (Guyton Physiology) 13th Edition by John E. Hall PhD
  2. Academia.dk
  3. ScienceDirect
  4. ResearchGate
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Salama has a degree in Life Science and Biochemistry from St. Xavier’s College, Mumbai. She enjoys being in the water much more than being on land. She is passionate about science and wants to declutter science from its jargon to make people understand its beauty.

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