The fermentation of chocolate is a process that takes place under low oxygen or anaerobic conditions with the help of microbes. Without these microbes, your chocolate would not be brown, nor would it have its characteristic flavor and aroma.
Nothing is more irresistible than the rich taste and smooth texture of a well-crafted chocolate bar. But what most people who enjoy chocolate don’t realize is that this extraordinary treat is brought to you by the concerted efforts of some very special microbes.
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Where does your chocolate come from?
The journey of chocolate begins with the Cacao tree, Theobroma cacao. These trees grow best in hot and humid regions, such as the West African countries of Ghana and the Ivory Coast, as well as Ecuador.

Cacao tree and Cacao pods (Photo Credit : Gstrau/Shutterstock)

Cacao pods and Cacao pulp-bean mass (Photo Credit : Aedka Studio/Shutterstock)
What is fermentation?
Fermentation is a metabolic process that takes place under either low oxygen or completely anaerobic conditions. Microbes, usually fungi like yeast and various bacteria, grow on a food stuff, altering the food’s composition by producing byproducts from their metabolism. Think of this process as a game of Telephone, where a message (a chemical) from one player (a microbe) gets modified and passed to another player (another microbe). Generally, fermentation comes in two types, homo and hetero fermentation; they differ based on the variety of the products formed. Homo-fermentation involves forming one type of product, as in the case of buttermilk, where lactic acid is the major product formed by certain bacteria. In hetero-fermentation, on the other hand, several products like acids and alcohols are formed, which is what occurs in cacao fermentation.
Fermentation of chocolate
1. The Anaerobic Phase Cocoa pulp-bean mass is stored in huge heaps and then covered with banana leaves, creating a low-oxygen environment. This enables appropriate growth conditions for beneficial microbes like yeast and Lactic Acid Bacteria (LAB). Yeast from the genera Candida, Hanseniaspora, Pichia, and Saccharomyces are the opening act of fermentation. Under anaerobic conditions and at temperature ranges of 25-30℃, yeasts consume the carbohydrate-rich pulp of cocoa beans, converting them into glucose and fructose. The breakdown of glucose (glycolysis) makes pyruvate, which is then converted to ethanol, carbon dioxide and other flavor precursors like esters or higher alcohols.

Fermentation of Cacao seeds (Photo Credit : Liz Miller/Shutterstock)

Cacao seeds during aerobic phase (Photo Credit : Kaiskynet Studio/Shutterstock)
Final stage of preparing chocolate
In order to make chocolate, cacao beans still need to undergo certain processes, like heating at high temperatures (roasting), separating the outer shell of beans (winnowing), grinding to decrease the grainy texture of fermented beans, conching (increasing texture and viscosity of the mixture) and molding to give them a desired structure.

Chocolate making in industries (Photo Credit :Macrovector/Shutterstock)
A Final Word
So why is this microbial-assisted process so essential? Researchers Van Thi Thuy Ho, Jian Zhao and Graham Fleet performed experiments to check the effect of the presence and absence of these microbes; it was observed that these microbes are essential to produce a consistent quality of chocolate. In one of the tests, natamycin was used to suppress the growth of yeast in cacao beans; this caused the beans to turn out purple with a sour (acidic) taste. Similar studies were also done to test the importance of LAB and AAB during cacao fermentation. To conclude, it’s the presence and interplay of microbes that enhances chocolate’s overall flavor profile, without which it wouldn’t be nearly as delicious and addictive as the treats we have come to crave!
Suggested Reading
The Beak of the Finch: A Story of Evolution in Our Time
- Journal: FEMS microbiology reviews
- International Journal of food Microbiology (Link 1)
- International Journal of Food Microbiology (Link 2)
- International Journal of Food Microbiology (Link 3)
- Journal: Critical reviews in food science and nutrition
- Textbook: Food Microbiology, 3rd edition, Martin R Adams and Maurice O. Moss